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. Author manuscript; available in PMC: 2018 Apr 27.
Published in final edited form as: React Oxyg Species (Apex). 2016;1(2):157–164. doi: 10.20455/ros.2016.819

FIGURE 3. Effects of varying the concentrations of ATP and luciferin on B16-F10 melanoma cell-derived bioluminescence.

FIGURE 3

Bioluminescence was measured at 25.6°C for 5 min after adding samples derived from 160 (panels A and B) or 16,000 (panels C and D) B16-F10-luc-G5 cells (without zeocin selection) to the reaction mix containing 100 μg/ml of luciferin plus the indicated concentrations of ATP (panels A and C) or 0.5 mM ATP plus the indicated concentrations of luciferin (panels B and D). Data represent means ± standard deviation from 4 separate experiments with the unit of bioluminescence being total counts of photon emission over 5 min.