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. Author manuscript; available in PMC: 2018 Apr 27.
Published in final edited form as: React Oxyg Species (Apex). 2016;1(2):157–164. doi: 10.20455/ros.2016.819

FIGURE 6. Linear relationship between the number of B16-F10 melanoma cells added to the lung tissue and the bioluminescence intensity.

FIGURE 6

Bioluminescence was measured at 37°C for 5 min after adding samples (derived from the indicated number of zeocin-selected B16-F10-luc-G5 cells added to normal lung tissues) to the reaction mix containing 100 μg/ml of luciferin and 0.5 mM ATP. Data represent means from 3–4 separate experiments with the unit of bioluminescence being total counts of photon emission over 5 min.