FIGURE 10.

Autophagy inhibitor 3-MA abolishes the protective effects of rapamycin in fibroblasts. Fibroblasts were pretreated with 3-MA (2 mM) for 2 h before further treatments. (A) Representative images of western blot analysis of LC3A/B and p62 after different treatments. (B) Representative images of fibroblasts stably transfected with GFP-RFP-LC3 were treated with 3-MA and rapamycin. (C) Representative images of western blot analysis of Col1, Col3, and α-SMA after different treatments. (D) Representative images of flow cytometry and (E) quantitative analysis of cell cycle distribution after different treatments. The data are shown as the means ± SD of three independent experiments. ^∗P < 0.05.