Figure 1. Summary of personalized therapy design.

Using the MMTV-Myc mouse model, EMT and Papillary tumors were used in a proof of principle experiment to predict therapy. For all tumors RNA was collected and gene expression was surveyed. Signaling pathway predictions (red on, blue off) for key signaling pathways were calculated based on gene expression signatures. EMT tumors were then transplanted to the mammary glands of syngenic mice. Once tumors reached 6 mm in the largest dimension, therapy for EMT, control therapy based on papillary tumors and vehicle control therapy was initiated in each cohort of mice. EMT treatment was found to be effective and specific for EMT tumors. Papillary treatment was also shown to be effective and specific for papillary tumors (not shown).