Table 1.

Methods for identifying mite allergens in the discovery phase

Authors	Methods	PubMed ID	Allergena	Year
Aki et al. [28]	cDNA library screen	7622766	Der f 10	1995
Ferrandiz et al. [18]	1D SDS-PAGE/western blotting	8556562	Der s 1 and 2	1995
Fujikawa et al. [25]	cDNA library screen	8649452	Der f 14	1996
King et al. [6]	Chromatography (protease activity)	8876548	Der p 9	1996
Morgan et al. [16]	IEF, 1D SDS-PAGE/western blotting	9275144	Eur m 2	1997
Wey et al. [53]	Chromatography (mAB)	10592827	94kd IgE binding protein	1997
Le Mao et al. [24]	2D SDS-PAGE/western blotting	9802372	Der f 14, Der f 15	1998
Tsai et al. [61]	Chromatography (mAB)	9723675	Der f 11	1998
Yi et al. [15]	1D SDS-PAGE/western blotting	10697258	Blo t IgE binding proteins	1999
Binder et al. [44]	cDNA library screen	11673567	Plo i 1	2001
McCall et al. [62]	1D SDS-PAGE/western blotting	11292526	Der f 15	2001
Weber et al. [37]	1D SDS-PAGE/western blotting	12847483	Der f 18	2003
Huntley et al. [45]	2D SDS-PAGE/western blotting	15679630	Pso o 10, 11 and 14	2004
Angus et al. [31] and Gao et al. [63]	EST screen	15651897	Blo t 21	2004
Harris et al. [21]	Protease activity screen	15489163	Der p 1	2004
Weghofer et al. [29]	cDNA library screen	18445190	Der p 21	2008
Weghofer et al. [30]	cDNA library screen	23460742	Der p 23	2013
An et al. [27]	Chromatography, 1 and 2D SDS-PAGE/western blotting	23481662	Der f 25, 28, 29, 30	2013
Chan et al. [32]	Sequence mining, 2D SDS-PAGE/western blotting	25445830	Der f 24	2015
Martins et al. [26]	IEF, 1 and 2D SDS-PAGE/western blotting	26015775	Der p IgE binding proteins	2015
Choopong et al. [43]	1 and 2D SDS-PAGE/western blotting	26754146	Aconitate hydrase	2016

^aIf a known allergen was identified in an unbiased screen, this was still considered a discovery attempt