Table 2.
Methods for identifying mite allergens in the confirmation phase
| Authors | Protein type | Expression system | Method of isolation | % Patients with IgE binding | Test for allerginicity (% Positive) | PubMed ID | Allergen | Year |
|---|---|---|---|---|---|---|---|---|
| Ferrandiz et al. [18] | N | Affinity chromatography (mAB) | 80–91 | NA | 8556562 | Der s1 Der s2 | 1995 | |
| King et al. [6] | N | Chromatography (protease activity) | 92 | NA | 8876548 | Der p 9 | 1996 | |
| Fujikawa et al. [25] | N | Affinity chromatography (AB) | Der f 14 | 1996 | ||||
| Wey et al. [53] | N | Affinity chromatography (mAB) | 37.50 | Skin prick (45%) | 10592827 | Der p 94 kD | 1997 | |
| Tsai et al. [61, 64] | N | Affinity chromatography (mAB) | > 80 | NA | 9723675 | Der f 11 Der p 11 | 1998 | |
| Olsson et al. [49] | N and R | E. coli and Baculovirus | Affinity chromatography (His-tagged) | Basophil activation | 9756203 | Lep d 2 | 1998 | |
| Kawamoto et al. [5] | R | E. coli | Affinity chromatography (GST-tagged) | 39 | Basophil activation | 10381565 | Der f 6 | 1999 |
| Binder et al. [44] | R | E. coli | Affinity chromatography (His-tagged) | 25 | Basophil activation skin prick | 11673567 | Plo i 1 (group 20 homologue) | 2001 |
| Cheong et al. [48] | R | E. coli | Affinity chromatography (GST-tagged) | 50 | Skin prick | 12708986 | Blo t 3 | 2003 |
| Ramos et al. [52] | N | Affinity chromatography (mAB) | 63 | NA | 15080814 | Blo t 1 | 2004 | |
| Cai et al. [65] | R | E. coli | Affinity chromatography (His-tagged) | NA | 17639694 | Der f 3 | 2007 | |
| Gao et al. [63] | R | E. coli | Affinity chromatography (His-tagged) | 58.00 | Skin prick | Blo t 21 | 2007 | |
| Weghofer et al. [29] | R | E. coli | Ion exchange chromatography (no tag) | 26 | Basophil activation | 18445190 | Der p 21 | 2008 |
| Weghofer et al. [66] | R | E. coli | Ion exchange chromatography (no tag) | 31 | Basophil activation | 18520154 | Der p 5 | 2008 |
| Beckham et al. [33] | R | E. coli and P. pastoris | Affinity chromatography (His-tagged) ion exchange chromatography | NA | NA | 19812030 | Sar s 3 | 2009 |
| Cui et al. [67] | R | E. coli | Affinity chromatography (His-tagged) | NA | NA | 19951588 | Der f 3 | 2009 |
| Cui et al. [68] | R | E. coli | Affinity chromatography (His-tagged) | NA | NA | 20939383 | Der f 7 | 2010 |
| Bordas-Le Floch et al. [50] | N and R | E. coli. and P. pastoris | Ion exchange chromatography (no tag) | NA | Basophil activation and mouse model | 22286395 | Der p 2 | 2012 |
| Weghofer et al. [30] | R | E. coli | Hydrophobic interaction and ion exchange chromatography (no tag) | 74 | Basophil activation | 23460742 | Der p 23 | 2013 |
| An et al. [27] | N | Chromatography (gel filtration and ion exchange) | 63–86 | Skin prick (60–70%) and basophil activation | 23481662 | Der f 25 Der f 28–30 | 2013 | |
| Banerjee et al. [4] | N and R | E. coli | Affinity chromatography (His-tagged or AB) | 5–67 | NA | Der p 11 | 2015 | |
| Lin et al. [69] | R | E. coli | Affinity chromatography (His-tagged) | NA | Skin prick (42.1%) and mouse model | 26623108 | Der f 27 | 2015 |
| Chan et al. [32] | R | E. coli | Affinity chromatography (His-tagged) | 100 | Skin prick (50%) | 25445830 | Der f 24 | 2015 |
| Cui et al. [70] | R | E. coli | Affinity chromatography (His-tagged) | 41 | NA | 26842967 | Der f 4 | 2016 |
| Lin et al. [35] | R | E. coli | Affinity chromatography (His-tagged) | NA | Skin prick (24.3%) | 27158348 | Der f 29b | 2016 |
R: recombinant. N: native. NA: not attempted or too few patients to draw conclusions about prevalence