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. 2018 Apr 27;37:90. doi: 10.1186/s13046-018-0762-y

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — The critical sites of HSP27 phosphorylation for AR cytoplasmic/nuclear translocation. Verification of deleting the residues of HSP27 phosphorylation sites (serine 15, 78, and 82) was measured by western blot (a). The expression and location of AR and HSP27 in the cytoplasm and nucleus after HSP27 phosphorylation sites deleted and treated with DHT were analyzed by western blot (b) and immunofluorescence (c) assays