Table 1.
Primers were designed to amplify the Full-length genome of PTV HuN-1
| Primers | Nucleotide Sequences | Position | Length(nt) |
|---|---|---|---|
| P1-F | 5′-CTCCCTTTGAATTTGTAA-3′ | 1–18 | 382 |
| P1-R | 5′-GGCCAGCCGCGACCCTGTCAG-3′ | 362–382 | |
| P2-F | 5′-GGACTGGACTTGTGCTGCC-3′ | 344–362 | 1328 |
| P2-R | 5′-ATRTCAACRCTRGGTGTTCCTCC-3′ | 1648–1671 | |
| P3-F | 5′-CCCTAGGACAAATTCMTCAGCAA-3′ | 1522–1544 | 978 |
| P3-R | 5′-CCTGTTTCTGCAGGTTGMAGGGG-3′ | 2477–2499 | |
| P4-F | 5′-GGTCACGGGGATACATCACTA-3′ | 2316–2336 | 988 |
| P4-R | 5′- AACAGGGAGAAGTTTGTAGCA −3′ | 3262–3282 | |
| P5-F | 5′-ATCTTATTCAAATGAATCTAC-3′ | 3172–3192 | 797 |
| P5-R | 5′-YTCTGTCTGGRTGCCTGTAATT-3′ | 3947–3968 | |
| P6-F | 5′- CGGCTCAAAACCTGGAGAACT −3′ | 3839–3859 | 789 |
| P6-R | 5′- TAGGGTATGTTTGCCATGTTTA −3 | 4606–4627 | |
| P7-F | 5′-AAGCCTGACGGGACACTTGAT-3 | 4493–4513 | 1457 |
| P7-R | 5′-GGTTCAAGAAAGTCTGGTGGC-3 | 5929–5949 | |
| P8-F | 5′- GCCAACATTTGTGTGTGGTGATC -3 | 5743–5765 | 1356 |
| P8-R | 5′-AACTAAAACTACTCAAGCAACAGGC-3 | 7074–7098 |