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Transcript level of 1-SST in the base of the growing leaf, estimated by RT-PCR. Eight 1-cm-long segments were cut, starting from the base of each growing leaf, and the total RNA was isolated from each segment. A DNA fragment of 520 bp was amplified by RT-PCR from each sample (lanes 1–8) reflecting relative amounts of the 1-SST message (A), and analyzed by gel electrophoresis. As a control a 258-bp fragment of the rRNA was amplified from each sample (B).
