Figure 7.

Characterization of the recombinant tall fescue 1-SST produced by P. pastoris. A, Time course over a period of 0 to 210 min. 1-SST was incubated with 100 mm Suc as the substrate, and production of Fru, 1-kestose, and nystose was measured. B, Saturation experiment. Recombinant 1-SST was incubated for 60 min with Suc as the substrate with concentrations ranging from 0 to 1,000 mm. Fru, 1-kestose, and nystose production was measured. C, Influence of temperature on enzyme specificity. Enzyme preparation was incubated with 100 mm Suc under the following conditions: 0°C for 60 min, 0°C for 240 min, and 27°C for 60 min. Production of Fru and 1-kestose was determined.