Table I.
Activities of enzyme produced by P. pastoris, incubated with Suc (100 mm), 1-kestose (50 mm), a combination of the two substances (100 and 50 mm, respectively), nystose (50 mm), or barley fructan (5% [w/v], DP 10–30)
| Substrate | Product Formation
|
|||
|---|---|---|---|---|
| Fru | Suc | 1-Kestose | Nystose | |
| nkat mL−1 | ||||
| Suc | 0.490 (±0.002) | a | 9.065 (±0.278) | 0.103 (±0.006) |
| 1-Kestose | 4.277 (±0.088) | 3.399 (±0.050) | a | 1.352 (±0.025) |
| Suc and 1-kestose | 0.875 (±0.023) | a | a | 0.468 (±0.005) |
| Nystose | 0.672 (±0.021) | 0.090 (±0.003) | 0.788 (±0.022) | a |
| Barley fructan (DP 10–30) | b | b | b | b |
Data are given in nkat per milliliter of the enzyme preparation.
a
Not measured (added as a substrate).
b
No product formation detectable on HPLC diagram (not analyzed quantitatively).