Table 4.

Comparisons between chemiluminescence and ORP.

Variable	Chemiluminescence	ORP
Type of sample [7], [21]	Only fresh sample Whole seminal ejaculate in an unprocessed or processed sample; sperm preparation by swim-up procedure and density gradient	Can measure in both fresh and frozen samples
Time to complete the test [7], [21]	Up to 60 min from start to finish. 15 min of actual run time and another 30 min to prepare the samples (control, blank, negative, test and positive sample), enter the information, print and calculate final ROS value.	Up to 4 min from start to finish
Sample volume [7], [21]	Minimum of 800 µL of sample in duplicate	High reproducibility; requires only 30 µL A single aliquot is adequate
Costs [7], [21]	Around $30,000	Ranges around $30 per sensor (sample)
Confounding variables [7], [21]	The specific type of luminometer instrumentation; single vs multiple tube, semen age, viscosity, presence of leucocytes and other nonspecific interference	Severe oligozoospermia (<2 × 106 sperm/mL) and viscous samples