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. 2018 Apr 27;18:475. doi: 10.1186/s12885-018-4385-7

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Upregulation of CR in Bt-exposed immortalized mesothelial (Met-5A) and MM cells a Upper panel: CR Western blot of cytosolic proteins from MSTO-211H cells treated with Bt for 72 h; left lane (CR): human recombinant CR (40 ng) as control. Lower panel: Ponceau Red-stained membrane to check for even loading. Ticks on the left mark the position of marker proteins (in kDa, from top to bottom: 75, 35, 28, 10). The signal for CR is slightly above the 28-kDa marker protein. b Western blots for CR of Bt-treated (72 h) cells: one mesothelial cell line (Met-5A) and 4 MM cell lines: ZL55, ZL5, SPC212, ZL34. Equal loading was confirmed by Ponceau Red staining as shown in a (not shown). c Semi-quantitative CR Western blot results (Mean ± S.D.) from 5 independent representative experiments. *** p < 0.001