Fig. 3.

Proteins differentially bound to the BRE9–13 DNA fragment a Silver-stained gel of proteins eluted from the BRE9–13 DNA-containing column from control MSTO-211H cells (−) or from Bt-treated (1 mM) cells (+) for 72 h; the region between 35 and 50 kDa (black bar) showed the strongest signals. A band was clearly weaker in extracts from Bt-treated cells (arrow). b Differentially expressed proteins listed (from top to bottom) according to their abundance in the samples analyzed by peptide shotgun MS. c Western blot for annexin A1 (top) and septin 7 (bottom) of either total cytosolic proteins (left) or of the proteins bound to the BRE9–13 DNA (right). d Binding assay of MSTO-211H soluble whole cell extracts with either a DNA fragment containing BRE5–6 or BRE9–13 identified as the septin 7-binding region of the CALB2 promoter. Eluate E1 was obtained after low stringency conditions, E2 under high stringency conditions