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. 2000 Nov;124(3):1229–1238. doi: 10.1104/pp.124.3.1229

Figure 1.

Figure 1

Expression of the ABP1 transgenes. A, Northern blot showing maize ABP1 mRNA accumulation in primary transformants. Blots were hybridized sequentially to a maize ABP1 probe (ABP1) and the constitutively expressed pCNT 6 (Memelink et al., 1987; control). The larger size of KDEL-ABP1 mRNA compared with the mutated ABP1 mRNAs is due to the (intentional) loss of some 3′-untranslated region during the in vitro mutagenesis schedule. The maize ABP1 probe did not detect wild-type tobacco ABP1 in untransformed plants (WT). B, Representative immunoblot showing expression of ABP1 in selected transgenic plants. Total microsomal protein loading ranged from 1 to 8 μg, as indicated, for the transgenic lines and 20 μg for microsomal protein from a wild-type (untransformed) tobacco plant (WT). ABP1 (250 ng) from maize microsomes was used as a positive control (maize control).