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. 2018 Apr 9;9:271. doi: 10.3389/fphys.2018.00271

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Copyright © 2018 Rathinasabapathy, Bryant, Suzuki, Moore, Shay, Gladson, West and Carrier.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Pulmonary macrophage dynamics in group 3 PH animals. Lung sections were stained for CD68 and random fields photographed at 10X and CD68⁺ cells counted (A,B, n = 4). RNA was isolated from frozen lungs and RT-PCR used to assess the expression of markers of macrophage polarization. (C) Retnla (Fizz1), (D) MRC1, (E) Arg1. Data presented in (C–E) are mean ± SEM, n = 4, 5, 6, and 6 for the control, ACE2, Bleo and Bleo+ACE2 groups respectively. Bleomycin-treated groups significantly differed from control groups by two-way ANOVA followed by Holm-Sidak's post-test, p < 0.0001 in (B–E). Scale bars shown are 50 microns.