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. 2018 Apr 24;6:e4664. doi: 10.7717/peerj.4664

Figure 2. Optimization of homogenization regime.

Figure 2

Single Achilles tendons and pools of four tendons were subjected to different durations of bead beating homogenization: 30, 60, 180, and 360 s (in two rounds of 180 s). The electropherogram digital gel shows that the longest beating time resulted in the most intact RNA, as evidenced by the strong 28s and 18s bands with 360 s (A). RIN values called by Agilent software also show the improvement in quality with longer beating time (B).