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. 2018 Apr 24;6:e4664. doi: 10.7717/peerj.4664

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© 2018 Grinstein et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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C_T standard deviations for Scx (A) and Gapdh (B) measurements were calculated for the technical replicates (n = 3 repeat measurements; dark gray) and for biological replicates (n = 3 independent samples; light gray), separately. ΔC_T was calculated by normalizing Scx C_T values to Gapdh. Technical (dark gray) and biological (light gray) variance estimates were calculated separately (C). All measures (A–C) show that biological variance increases as number of individuals contributing to a pool increases.