Table 2. Purity measurements for different homogenization methods and single versus pooled tendon samples.

(A) RNA purity (260/280 ratio), concentration, and RIN measurements are poor following Polytron or Spex freezer mill homogenization methods
Sample	Number of tendons	Conc. (ng/μl)	Homogenization method	RIN	260/280	260/230
8wk poly 1	One	32.5	Polytron	None	1.57	0.49
8wk poly 2	One	51	Polytron	2.5	1.62	0.34
8wk poly 3	Three	71.2	Polytron	2.6	1.58	0.33
8wk poly 4	Three	29.4	Polytron	None	1.56	0.65
8wk spex 1	Four	170	Spex freezer mill	2.4	1.44	0.22
8wk spex 2	Four	80	Spex freezer mill	2.4	1.47	0.25
8wk spex 3	One	4.4	Spex freezer mill	2.3	1.44	0.31
8wk spex 4	One	4.7	Spex freezer mill	None	2.26	0.01