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. Author manuscript; available in PMC: 2019 Apr 15.
Published in final edited form as: J Immunol. 2018 Mar 19;200(8):2927–2940. doi: 10.4049/jimmunol.1701417

FIGURE 6. SHS induces defect in the generation of adaptive immune responses against NTHI-mediated chronic respiratory infection by impairing the frequencies of antigen-specific cytokine secreting T- and Ab producing B- cells.

FIGURE 6

Single-cell suspensions of (A) lung lymphocytes and (B) splenocytes were isolated from air- or SHS-exposed mice and incubated with P641–55 peptide-pulsed APCs to measure the frequency of cytokine-secreting T cells by ELISPOT (n = 10). B cell ELISPOTs were performed to quantify the numbers of P6-specific (C) IgG1- and (D) IgG2a-secreting cells from bone marrow (left panels) and spleens (right panels) of air- or SHS-exposed mice (n = 10). Spots were developed with TMB substrate and counted using a CTL ELISPOT reader. Line represents mean. **p≤0.01, ***p≤0.005, ****p≤0.0001 two tailed unpaired t test.