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. Author manuscript; available in PMC: 2018 Apr 27.
Published in final edited form as: J Immunol. 2014 Oct 22;193(11):5751–5757. doi: 10.4049/jimmunol.1402059

Figure 3. Selection of Class I SLA negative Fetal Fibroblast Cells.

Figure 3

SCNT of fibroblasts isolated in figure 2 were used to create embryos. 32 days after impregnating a sow with these embryos, three fetuses were collected. Two of the fetuses were well formed and used to create fibroblast cultures. The fibroblasts were stained with a negative isotype control or with and antibody specific for class I SLA. Fetus-3 expressed low levels of SLA protein. Cells derived from Fetus-2 were devoid of class I SLA proteins.