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. 2018 Mar 8;3(5):e97018. doi: 10.1172/jci.insight.97018

Figure 4. Inferring the presence of CD4bs bnAbs via STG-based enrichment and serum profiling.

Figure 4

A set of 16 samples from the RV217 HIV cohort were titrated for binding to WT (red) and STG (blue) core before and following enrichment of CD4bs bnAbs via STG-based depletion to enable inference of the presence or absence of CD4bs bnAbs. VRC01, PGV04, or F105 were spiked into HIV immune globulin (HIVIG) as positive and negative controls. (A) Titration curves of VRC01-spiked and F105-spiked HIVIG (top), and two RV217 subjects (bottom) before (broken line) and after (solid line) enrichment. The dotted line indicates the signal baseline used for AUC calculations. (B) The AUC for all sample titrations against WT and STG cores after enrichment was calculated and samples were plotted by rank in the ratio of the AUC for WT relative to STG. The mAb-spiked samples are highlighted in gray. Samples with no measurable binding were assigned a value of 100.