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. 2018 Apr 23;13:2491–2505. doi: 10.2147/IJN.S157935

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© 2018 Lu et al. This work is published and licensed by Dove Medical Press Limited

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qRT-PCR and Western blot analyses of U251 cells after different treatments.

Notes: (A) The quantitative real-time polymerase chain reaction analyses to evaluate the relative mRNA levels of caspase-3, caspase-8, and caspase-9 in U251 cells with various treatments and (C) β-actin was used as an internal control to normalize the data. (B) Western blot analyses to evaluate the protein levels of caspase-3, caspase-8, and caspase-9 in U251 cells of different treatment groups, and (D) β-actin was used as an internal control to normalize the data.

Abbreviations: MFH, magnetic fluid hyperthermia; MNPs, magnetic nanoparticles; NIR, near-infrared hyperthermia.