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. 2018 Apr 10;9(27):18665–18681. doi: 10.18632/oncotarget.24816

Figure 4. Effect of different electrotransfer pulse protocols on fold changes in mRNA and protein levels of INFβ and TNFα.

Figure 4

Levels of IFNβ and TNFα mRNA (A, B) were determined 4 hours after electrotransfer of vector pDNA using the pulse protocols described in methods and Figure 1. Intracellular levels of IFNβ and TNFα in the supernatant (C, D) were measured by ELISA 4 hours after electrotransfer.*statistically significant difference compared to electrotransfer protocol only (EP) groups (p < 0.05).