Table 2. Fold changes in mRNA levels of endosomal and cytosolic DNA sensors in WEHI 164 tumor cells 4 hours after pDNA electrotransfer.
| WEHI 164 | control | gWiz Blank | EP 1 | EP 2 | EP 3 | pDNA+EP1 | pDNA+EP2 | pDNA+EP3 | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| n | foldex ± SE | n | foldex ± SE | n | foldex ± SE | n | foldex ± SE | n | foldex ± SE | n | foldex ± SE | n | foldex ± SE | n | foldex ± SE | |
| TLR9 | 4 | / | 3 | / | 3 | / | 3 | / | 3 | / | 5 | / | 5 | / | 3 | / |
| RIG-1 | 4 | / | 3 | / | 3 | / | 3 | / | 3 | / | 5 | / | 5 | / | 3 | / |
| DDX60 | 4 | 1.0 ± 0.1 | 3 | 1.3 ± 0.3 | 3 | 1.4 ± 0.2 | 3 | 1.0 ± 0.3 | 3 | 0.4 ± 0.1 | 5 | 16.4 ± 5.4* | 5 | 58.1 ± 22.4* | 3 | 1.9 ± 0.2 |
| DHX9 | 4 | 1.1 ± 0.3 | 3 | 1.3 ± 0.1 | 3 | 1.8 ± 0.4 | 3 | 1.6 ± 0.2 | 3 | 0.8 ± 0.1 | 5 | 2.0 ± 0.3 | 5 | 1.0 ± 0.2 | 3 | 1.0 ± 0.1 |
| DHX36 | 4 | 1.0 ± 0.1 | 3 | 1.4 ± 0.5 | 3 | 1.9 ± 0.3 | 3 | 1.3 ± 0.1 | 3 | 0.5 ± 0.2 | 5 | 1.0 ± 0.4 | 5 | 1.1 ± 0.3 | 3 | 0.7 ± 0.1 |
| AIM | 4 | / | 3 | / | 3 | / | 3 | / | 3 | / | 5 | / | 5 | / | 3 | / |
| cGAS | 4 | 1.0 ± 0.1 | 3 | 1.1 ± 0.0 | 3 | 2.4 ± 0.3 | 3 | 1.6 ± 0.1 | 3 | 1.0 ± 0.1 | 5 | 0.7 ± 0.2 | 5 | 1.2 ± 0.4 | 3 | 1.9 ± 0.2 |
| DAI/ZBP1 | 4 | 1.0 ± 0.2 | 3 | 1.4 ± 0.4 | 3 | 1.4 ± 0.3 | 3 | 1.3 ± 0.1 | 3 | 0.5 ± 0.0 | 5 | 3.4 ± 0.1* | 5 | 27.2 ± 1.8* | 3 | 3.1 ± 0.3* |
| DDX41 | 4 | 1.0 ± 0.1 | 3 | 1.1 ± 0.2 | 3 | 1.3 ± 0.2 | 3 | 1.1 ± 0.1 | 3 | 0.7 ± 0.1 | 5 | 1.5 ± 0.3 | 5 | 1.4 ± 0.1 | 3 | 0.9 ± 0.1 |
| LRRFIP1 | 4 | 1.1 ± 0.2 | 3 | 1.2 ± 0.3 | 3 | 1.8 ± 0.5 | 3 | 1.8 ± 0.1 | 3 | 0.8 ± 0.1 | 5 | 1.5 ± 0.1 | 5 | 2.7 ± 0.1* | 3 | 1.0 ± 0.2 |
| P202 | 4 | 1.0 ± 0.2 | 3 | 1.2 ± 0.2 | 3 | 1.5 ± 0.3 | 3 | 1.2 ± 0.2 | 3 | 0.9 ± 0.1 | 5 | 1.7 ± 0.5 | 5 | 3.7 ± 0.3* | 3 | 0.9 ± 0.2 |
| P204 | 3 | 1.0 ± 0.0 | 3 | 1.3 ± 0.2 | 3 | 1.0 ± 0.0 | 3 | 1.0 ± 0.1 | 3 | 1.1 ± 0.1 | 3 | 2.5 ± 0.7 | 3 | 2.6 ± 0.2* | 3 | 1.3 ± 0.2 |
| SOX2 | 3 | 1.0 ± 0.3 | 3 | 1.0 ± 0.3 | 2 | 1.0 ± 0.3 | 2 | 1.4 ± 0.1 | 2 | 0.4 ± 0.0 | 2 | 0.5 ± 0.1 | 2 | 0.5 ± 0.2 | 2 | 0.9 ± 0.2 |
| MRE1 | 3 | 1.0 ± 0.2 | 3 | 0.9 ± 0.2 | 2 | 1.8 ± 0.6 | 2 | 0.9 ± 0.0 | 2 | 0.6 ± 0.0 | 2 | 1.1 ± 0.1 | 2 | 0.8 ± 0.1 | 2 | 1.0 ± 0.1 |
| Ku70 | 3 | 1.0 ± 0.2 | 3 | 0.9 ± 0.1 | 2 | 1.9 ± 0.7 | 2 | 0.8 ± 0.1 | 2 | 0.8 ± 0.0 | 2 | 1.2 ± 0.1 | 2 | 0.7 ± 0.1 | 2 | 1.2 ± 0.0 |
Legend: cGAS, cyclic guanosine monophosphate-adenosine monophosphate synthase; DAI/ZBP1, DNA-dependent activator of interferon regulatory factor; DDX41, DEAD (Asp-Glu-Ala-Asp) box polypeptide 41; DDX60, DEAD (Asp-Glu-Ala-Asp) box polypeptide 60; DHX9, DEAH (Asp-Glu-Ala-His)box helicase 9; DHX36, DEAH (Asp-Glu-Ala-His) box helicase 36; LRRFIP1, leucine-rich repeat flightless-interacting protein 1; p204, interferon activated gene 204; p202, interferon activated gene 202; MRE11, meiotic recombination 11 homolog, double strand break repair nuclease; SOX2, SRY (sex determining region Y)-box 2; Ku70, Lupus Ku autoantigen protein p70*statistically significant difference compared to control group (P < 0.05); ND, not detected.