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. 2018 Apr 10;9(27):18949–18969. doi: 10.18632/oncotarget.24855

Figure 1. Human cancer cells display a strong inverse correlation between HK1 and HK2 expression.

Figure 1

(A) Western blotting of HK1 and HK2 in several cancer cell lines. Total protein extracts isolated from cells as indicated were blotted with antibodies specific for HK1, HK2 and β-actin. The β-actin level serves as the loading control for total proteins. (B) Immunofluorescent staining of HK1 and HK2 in various cancer cell lines. Cells as indicated were stained with antibodies specific for HK1 and HK2; nuclei were counterstained with DAPI. (C) Western blotting of HK1 and HK2 in cellular fractions isolated from HeLa cells. Cytosolic (C) and mitochondrial (M) isolates prepared from HeLa cells were blotted with antibodies specific for protein as labelled. The α-tubulin and cytochrome C (Cyt-C) serve as markers specific for the cytosolic and mitochondrial compartments, respectively.