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. 2018 Apr 10;9(27):18949–18969. doi: 10.18632/oncotarget.24855

Figure 4. EMT phenotype caused by HK1 silencing increases cancer cell proliferation.

Figure 4

(A) MTT cell growth assay of HK1-knocked down cells. Cells as indicated were seeded in 96-well plates, incubated for various time periods as labelled, then the MTT cell growth assay was performed according to standard protocols. (B) BrdU incorporation assay of HK1-silenced cells. Cells as indicated were loaded into 96-well plates and incubated for 3 days, then the BrdU (colorimetric) cell proliferation ELISA was carried out according to the manufacturer’s procedures. (C) Colony formation assay of HK1-inhibited cells. Cells as indicated were cultured in 6-well plates for 6 days. Colonies were fixed, stained, imaged and enumerated. (D) Soft agar colony formation assay of HK1-knocked down cells. Cells as indicated were suspended in 0.35% top agar and overlaid on 0.7% bottom agar, then cultured in 6-well plates for 12 days. Colonies were directly imaged and enumerated. The plotted data are averaged from three independent experiments and the bars represent mean ± SD.