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. 2018 Apr 10;9(27):18949–18969. doi: 10.18632/oncotarget.24855

Figure 9. Silencing of HK1 enhances glucose dependence during cell growth.

Figure 9

(A) MTT cell growth assay of HK1-knocked down cells cultured in various glucose concentrations. Cells as indicated were seeded in 96-well plates with culture media containing 4.5, 1 and 0 mg/ml glucose. After incubation for various time periods, an MTT cell growth assay was performed according to standard protocols. (B) Colony formation assay of HK1-silenced cells cultured in different glucose concentrations. Cells as indicated were cultured in 6-well plates with media containing 4.5, 1 and 0 mg/ml glucose for 6 or 10 days. After incubation, colonies were stained, imaged and enumerated. * indicates incubation for 10 days. (C) Cell apoptosis assay of HK1-knocked down cells cultured in distinct glucose concentrations. Cells as indicated were grown in 6-well plates with media containing 4.5, 1 and 0 mg/ml glucose for 3 days. After incubation, cells were collected, fixed and subjected to a cell apoptosis assay using the FITC Annexin V Apoptosis Detection Kit I according to the manufacturer’s procedures. Fluorescence intensities of propidium iodide and annexin V-FITC stained cells were analysed using a flow cytometer. The plotted data are averaged from three independent experiments and the bars represent mean ± SD.