Table 1. HMGB1 preys in HOSEpic and HPEpiC cells.

Gene	N	Cells	Function	Relation to cancer
ACBD3	7	HOSEpiC	Apoptosis	Golgi resident protein GCP60 (GOCAP1) can interact with a golgin-160 fragment to regulate cell apoptosis [58].
C1QBP	2	HOSEpiC	Cell motility	C1qBP protects the cells against staurosporine induced apoptosis and increases proliferation and cell migration in cancerous cells [59]. Predictor of tumor size in progesterone positive tumors and marker for proliferation in breast cancer [60].
CCAR1	3	HOSEpiC	Apoptosis	Cell division cycle and apoptosis regulator protein 1 (CARP-1) regulates cell growth and apoptosis by serving as a co-activator of several genes including steroid nuclear receptors and tumour suppressor p53 [49].
CTNNBL1	1	HOSEpiC	Tumour progression	The spliceosome-associated factor, β-catenin-like protein 1, is overexpressed in high-grade serous ovarian carcinomas. Moreover, this protein can promote proliferation and invasion in ovarian cancer cells [29].
EIF1/SUI1	1	HPEpiC	Apoptosis	Eukaryotic translation initiation factor 1 (eIF1) is activated by oncogene MCT-1 [61].
ENAH	2	HPEpiC	Cellular signalling	Protein enabled homolog. Involved in PI3K-dependent cell invasion induced by Platelet-derived growth factor in human breast cancer cells [62]. Involved in human lung cancer metastasis and migration of breast cancer cells [63].
FIP1L1	1	HPEpiC	−	The fusion of the gene encoding the Pre-mRNA 3'-end-processing factor FIP, with the PDGFRA gene or the RARA gene generate a novel tyrosine kinase due to a interstitial chromosomal deletion [64]. These associations have been linked to the pathogenesis of leukemias [65].
HSPA5	1	HOSEpiC	Tumour progression	78 kDa glucose-regulated protein (GRP-78) or Heat shock 70 kDa protein 5 has been proposed as a potential biomarker for predicting high-risk endometrial carcinoma [66], as well as positive regulator of cellular migration [67].
KRT7	19	HPEpiC	Cytoskeleton	Keratin, type II cytoskeletal 7. The keratin KRT7 gene has been found to be hyper-methylated in prostate cancer [33].
NEXN	11	HOSEpiC	Cytoskeleton Cell motility	Nexilin is an F-actin associated protein that stimulates cell motility (Wang et al., 2005) and NEXN is down regulated after radiation of prostate adenocarcinoma cells [68].
PCOLCE	1	HOSEpiC	Tumour progression	Procollagen C-endopeptidase enhancer 1 (PCPE-1) binds procollagen, potentiating its cleavage by specific proteinases, and might be involved in tumour growth [69] .
RPL29	2	HOSEpiC	Tumour progression	60S ribosomal protein L29 has been related to tumoral events as angiogenesis and cell proliferation [70].
RPS20	1	HOSEpiC	Cellular signalling	40S ribosomal protein S20 binds the RING-type E3 ubiquitin ligases Mdm2 and MdmX therefore regulating the Mdm2-p53-MdmX network [71].
SPINT1	2	HPEpiC	Cellular signalling	Kunitz-type protease inhibitor 1 (or hepatocyte growth factor activator inhibitor type 1, HAI-1) malfunction promotes intestinal carcinogenesis by activating NF-κB signalling [72]. Low HAI-1 expression is a significant predictor for poor prognosis in prostate cancer [73].
UBE2I	1	HPEpiC	Tumour progression	SUMO-conjugating enzyme UBC9, plays a major role in sumoylation [74]. UBE2I expression is increased in several cancers as in epithelial ovarian cancer (EOC) [75], colon and prostate neoplasias and adenocarcinomas [76]. Moreover, overexpression of UBE2I has also been related to migration, invasion and proliferation in a lung cancer cell line [77] and in ovarian cancer [78]
ZNF428	11	HOSEpiC	Tumour progression	Zinc finger protein 428 interacts with Bcl2-associated athanogene 3 (BAG3) protein, which is related to cancerous processes. BAG3 can modulate apoptosis, autophagy, mechanotransduction, cytoskeleton organization, and motility [79]. Moreover, BAG3 regulates epithelial-mesenchymal transition (EMT) and angiogenesis and its knockdown reduced migration and invasion in cancerous cells [80].

N: repetitive identification during Y2H screening