Figure 5.

A, Puncture force analysis of wild-type MM seeds at different times after imbibition in water (○) or 100 μm ABA (●). Error bars indicate ± se (n = 24) when they exceed the size of the symbols. B, LeEXP4 mRNA abundance in MM seeds after imbibition in water or in 100 μm ABA. Total RNA extracted at various times after imbibition was separated by electrophoresis and hybridized with a LeEXP4-specific cDNA probe. Bottom sections in each pair show ethidium bromide-stained rRNA to indicate the relative RNA loading of each lane.