Figure 7.

LeEXP4 expression in wild-type MM seeds imbibed at different water potentials or under far-red light. A, Total RNA isolated from seeds imbibed for 40 h in water or in PEG 8,000 solutions of −0.5, −1.0, or −2.0 MPa was hybridized with a LeEXP4-specific probe. B, Total RNA isolated from seeds imbibed for 40 h in the dark (D) or under continuous far-red (FR) light was hybridized with a LeEXP4-specific probe. Lower sections in each pair show ethidium bromide stained rRNA to indicate the relative RNA loading of each lane. In A and B, the puncture force (PF) values (and se, n = 24) below each lane were recorded for endosperm caps of each treatment at the time that RNA samples were collected (40 h of imbibition).