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. Author manuscript; available in PMC: 2019 Apr 16.
Published in final edited form as: Analyst. 2018 Apr 16;143(8):1924–1933. doi: 10.1039/c7an01897e

Figure 3.

Figure 3

Modification of DENV1/3 BIP prevents self-extension in QUASR. (A) Original DENV1/3 BIP-Cy5 was incubated with or without BIPc-10 quencher (no other primers) in RT-LAMP assay with enzyme (solid trace) or without enzyme (dashed trace), indicating that the primer self-extends in the presence of enzyme, inhibiting quenching upon cooling down. For comparison, a reaction with no quencher is shown (short dashes) illustrating the full (unquenched) fluorescence of the primer. (B) DENV3 QUASR with modified DENV1/3 BIP-Cy5 primer, positive control (104 copies DENV3 RNA), or no-template control, displaying the expected behavior (quenching of no-template control). (C) Endpoint fluorescence images of original and modified (“bumped”) DENV3 QUASR reactions in PCR tubes. Positive controls have 5000 copies DENV3 RNA as a template.