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STAgR constructs allow simultaneous genetic deletions in vivo. Above: Imaging setup from whole mount adult zebrafish brains. Below: 3D reconstructions of whole mount Tg(gfap:GFP) zebrafish telencephali (depicted from above). GFP+ and Sox2+ ependymoglia have been electroporated with STAgR targeting GFP and Sox2 (above) or a vector control (below) together with a Cas9 expression vector. Scale bar represents 50μm.
