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. 2018 Apr 27;13(4):e0196469. doi: 10.1371/journal.pone.0196469

Fig 4. Expression of HSPs after heat shock treatment.

Fig 4

Cultured DFTD cells were heat shocked at 42 oC for 30 min in a water bath and then recovered in an incubator under normal cell culture conditions (35 oC) for 4, 8, or 24 hours. a. HSP gene expression was analysed using qRT-PCR. Expression was measured relative to the control gene RPS18. Results are the mean and standard error of three biological replicates. Significance is defined as * p<0.05, ** p<0.01 and *** p<0.001. b. Protein expression of HSPs was analysed using western blot. Expression was measured relative to the untreated control. Results are the mean and standard error of two biological replicates. Statistical significance is defined as * p<0.05, ** p<0.01 and *** p<0.001. Results are representative of two experiments.