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Effect of leaf detachment and plant hormones on the temporal pattern of SARK mRNA expression. A, Amounts of Chl in leaves (top) after treatment with water (black bars) or 0.1 mm BA (shaded bars) compared with SARK mRNA levels without or with BA. All treatments were in the dark. B, Analysis of SARK mRNA levels after incubation of detached leaves on water or 1 mm ACC. The membrane was probed with the radiolabeled SARK cDNA clone and rRNA was stained with methylene blue.
