Figure 3.
Effect of spermidine on IKin in guard cells. In V. faba guard cell protoplasts, whole-cell patch-clamp recordings revealed the typical IKin current (B), IKin current with 1 mm spermidine in pipette solution (C), and with 1 mm spermidine in bath solution (D). During the recordings, the holding potential was −50 mV, the currents were recorded at the membrane potentials from −20 to −160 mV with increment of −20 mV (A), and the current and time scales for B, C, and D are indicated by vertical and horizontal bars shown in D. The current amplitudes (mean ± se) from 14 control cells (○) and 18 cells treated with 1 mm spermidine in the pipette solution (●) are presented as current-voltage (I–V) curves in E. All data shown here were collected from whole-cell configuration with 2.5 GΩ or higher seal resistance. Spd, Spermidine.