Table 3. Sucrose density gradients used in this study.
| Media | Time | Sucrose range (Density)* |
Average density ±StDev |
High buoyancy fractions (Density) | Median buoyancy fractions (Density) | Low buoyancy fractions (Density) |
|---|---|---|---|---|---|---|
| Low Nitrogen | 40 hr | 50–20% (1.22–1.10) |
1.177 ±0.003 |
17–20 (<1.11) |
6–7 (1.18–1.19) |
1–2 (>1.21) |
| YPD | 40 hr | 80–50% (1.29–1.16) |
1.234 ±0.012 |
19–22†
(<1.14) |
4–8†
(1.24–1.27) |
1 (>1.28) |
All density measurements in g/mL.
*Highest and lowest specific density measured in any collected fraction in the linear portion of the gradient.
†Some biological replicates differ in exact fractions collected. Fractions were collected within this range such that the high buoyancy fraction constituted the most buoyant 5–10% of the population, the median buoyancy fraction constituted the median 30–50% of the population and the low buoyancy fraction constituted the least buoyant 5–10% of the population.