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. Author manuscript; available in PMC: 2018 Apr 27.
Published in final edited form as: Cell. 2016 Sep 8;166(6):1553–1563.e10. doi: 10.1016/j.cell.2016.08.042

Figure 4. Non-autonomous Induction of hsp-6p::GFP Requires unc-31 Mediated Neurosecretion.

Figure 4

(A) Photomicrographs depicting hsp-6p::GFP reporter response in rgef-1p::polyQ40::YFP; hsp-6p::GFP animals in combination with unc-31(e928), unc-13(e1091), or unc-18 (e81) mutations.

(B) Photomicrographs depicting unc-31(e928); rgef-1p::polyQ40::YFP; hsp-6p::GFP animals treated with 5 mM serotonin (5-HT) or with vehicle control.

(C) ImageJ quantification of the hsp-6p::GFP expression from unc-31(e928);rgef-1p::polyQ40:: YFP; hsp-6p::GFP animals after the application of serotonin, dopamine, octopamine, or tyramine (Mean ± SEM for n = 20 animals/experiment, p < 0.01 by Student’s t test for serotonin versus vehicle treatment).

(D) ImageJ quantification of hsp-6p::GFP fluorescence following the application of dopamine, octopamine, and tyramine to hsp-6p::GFP animals in the absence of PolyQ40 expression (Mean ± SEM for n = 20 animals/experiment, all conditions were n.s. compared to vehicle control).