Figure 1. Knockdown of Mitochondrial HSP70, hsp-6, Induces Cytosolic Heat Shock Response via UPRmt.
(A) hsp-6 and hsp-1 RNAi-induced cytosolic small heat shock protein expression. hsp-16.2p::GFP induction was measured by COPAS biosorter (bottom) (mean ± SD of three biological repeats; **p ≤ 0.01). EV, empty vector control RNAi.
(B) qPCR of UPRER and cytosolic HSR genes after hsp-6 RNAi (mean ± SD of three biological repeats).
(C) Cytosolic HSR induced by hsp-6 RNAi was dependent on both hsf-1 and UPRmt components. hsp-16.2p::GFP induction was measured by COPAS biosorter (bottom) (mean ± SD of three biological repeats; **p ≤ 0.001).
See also Figure S1 and Table S1.