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. 2018 Apr 23;9:833. doi: 10.3389/fimmu.2018.00833

Figure 6.

Figure 6

Effect of transforming growth factor (TGF)-β1 administration inflammation and pulmonary injury in septic neonatal mice. Neonatal C57BL/6 pups received recombinant mouse TGF-β1 (0.05 µg/g BW) or vehicle (PBS containing 0.8 mM HCl) intraperitoneal (i.p.) 1 h prior to sepsis induction. Sepsis was induced by i.p. injection of cecal slurry (0.9 mg/g BW); serum and lungs were harvested 10 h later. Serum levels of (A) IL-6 and (B) IL-1β were measured by enzyme-linked immunosorbent assay. The mRNA levels of (C) IL-6, (D) IL-1β, (E) keratinocyte chemoattractant, and (F) macrophage inflammatory protein-2 in the lungs were determined by qPCR. (G–I) Shown are representative hematoxylin and eosin-stained sections of the lung from (G) sham, (H) vehicle-, and (I) TGF-β1-treated mice at 200× magnification. (J) Histologic lung injury score was calculated for each group with a maximum possible score of 1. Data expressed as mean ± SEM (n = 7–8 per group) and compared by one-way analysis of variance (*p < 0.05 vs. Sham, #p < 0.05 vs. Vehicle).