Table 3.

Primer sequences and amplification length

Gene	Primer sequences	Amplification length
TNF-α	Forward: 5’-GCCTATGTCTCAGCCTCTTCTC-3’	22
	Reverse: 5’-TGGTGGTTTGCTACGACGTG-3’	20
CXCL-1	Forward: 5’-TCACCTCAAGAACATCCAGAGC-3’	22
	Reverse: 5’-ACTTGGGGACACCTTTTAGCAT-3’	22
IL-6	Forward: 5’-TCTCTGCAAGAGACTTCCATCC-3’	22
	Reverse: 5’-TTCCACGATTTCCCAGAGAACA-3’	22
β-actin	Forward: 5’-AGATCAAGATCATTGCTCCTCC-3’	22
	Reverse: 5’-GGTGTAAAACGCAGCTCAGTAA-3’	22

TNF-α: Tumor necrosis factor-alpha; CXCL-1: Chemokine (C-X-C motif) ligand-1; IL-6: Interleukin-6; β-actin: Beta-actin.