Figure 4.

CD3⁺CD4⁺CD25⁺Foxp3⁺ T cells are increased in mice treated with DSS and NaCl. A: CD3⁺CD4⁺CD25⁺Foxp3⁺ T cells in LP, MLN and SP from animal models were detected by flow cytometry; B: A summary of the percentages of CD3⁺CD4⁺CD25⁺Foxp3⁺ T cell distribution in LP, MLN and SP; C: LPMCs from the four groups were isolated and cultured for 24 h, and the levels of cytokines in the culture supernatants were collected and analyzed by enzyme-linked immunosorbent assay. In all the panels, data indicate three separate experiments, whereby 3 mice per group were used in each experiment. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001 vs the DSS group. DSS: Dextran sulfate sodium; LMPCs: Lamina propria mononuclear cells; LP: Lamina propria; MLN: Mesenteric lymph node; SP: Spleen.