Fig. 3.

NF-κB activation is involved in golgin-97-mediated cell migration and invasion. a qPCR analysis of the indicated significantly regulated genes in golgin-97-knockdown cells. The results are expressed as the mean ± SEM from triplicate experiments. *p < 0.05. b NF-κB p65 controls the significant network deduced from the 428 upregulated genes identified from the comparison between golgin-97-knockdown cells and control cells. Nodes represent gene names, and lines between nodes indicate protein-protein interactions, with green, red, and gray lines denoting positive, negative, and unspecified effects, respectively. Highlighted lines indicate canonical pathways. Global nodes highlight genes identified in the current study, and red bottoms indicate upregulated genes in golgin-97-knockdown cells. c Treatment with NF-κB inhibitor abolishes golgin-97-knockdown-induced cell migration. Representative microphotographs of filters were obtained from the migration/invasion assays. Original magnification: 100×. *p < 0.05. d Treatment of CM derived from golgin-97-knockdown cells promoted cell migration/invasion abilities of recipient cells. Western blot analysis was used to confirm the knockdown efficacy. Representative microphotographs of filters were obtained from the migration/invasion assays. Original magnification: 100×. e Quantitative analysis of the migration/invasion assays is presented as the mean ± SEM obtained from three independent experiments. *p < 0.05