Fig. 4.

Golgin-97 knockdown induces NF-κB activation by reducing IκBα levels. a Western blot analysis of nuclear entry of phospho-p65 in control (NC) or golgin-97 (G97)-knockdown cells. GAPDH and Lamin A/C were used as controls for cytosolic and nuclear fractions, respectively. b Quantification analysis of nuclear p65 and phospho-p65 acquired from western blot analysis. c NF-κB activation determined by luciferase reporter assay. d IκBα protein levels were reduced in golgin-97-knockdown cells. Actin was used as the internal control. Quantitative results are presented as the means±SEM from three independent experiments. *p < 0.05. ***p < 0.001. n.s., no significance