Fig. 5.

Loss of Golgi integrity is not involved in the golgin-97 knockdown-induced NF-κB activation. a Representative images of Golgi markers in monensin-treated MDA-MB-231 or HeLa cells. Scale bars, 10 μm. b Western blot analysis of Golgi markers in monensin-treated MDA-MB-231 or HeLa cells. Actin was used as the internal control. c Luciferase reporter assay of monensin-treated MDA-MB-231 or HeLa cells. Relative levels of NF-κB activation are presented as the mean ± SEM from three independent experiments. n.s., no significance