Fig. 2.
H19 is induced upon ATRA treatment in NB4-LR1 cells in association with hTERT repression, whereas it is repressed in the NB4-LR1SFD cells resistant to ATRA-induced hTERT repression. a Basal expression levels of H19, hTR, and hTERT mRNA and relative telomere activity (RTA) in NB4-LR1 and NB4-LR1SFD cells. b Expression levels of H19, hTR, and hTERT mRNA and RTA after treatment of NB4-LR1 and NB4-LR1SFD cells with ATRA (1 μM) for the indicated time. All experiments were repeated at least 3 times. Expression levels were normalized to GAPDH expression. RTA measured by qTRAP as described in “Methods” is expressed as the percentage of that detected in the untreated cells. Results were expressed as means +/− SEM. t-test (a) or one way ANOVA with post-hoc Tukey, ns p > 0.05, *p < 0.05, **p < 0.001, ***p < 0.001