FIG 2.

Cleaved caspase-3 in the intestines of mice following infection with reovirus T1L or T3D-RV. Mice were inoculated perorally with 10⁸ PFU of T1L or T3D-RV or PBS (mock). One day after inoculation, intestines were resected. The distal half was flushed, Swiss rolled, and processed for histology. (A) Sections were stained with H&E, reovirus polyclonal antiserum, or antibody against cleaved caspase-3. Representative sections of jejunum are shown (scale bar, 100 μm). (B) Cells positive for cleaved caspase-3 were enumerated manually and normalized per villus. Each symbol represents an individual mouse (n = 5 to 18 mice per group). (C) Cleaved-caspase-3 staining in the lumen was quantified by outlining the luminal region using the Digital Histology Shared Resource tool (n = 3 mice per virus). The percent luminal staining was determined as follows: (area in the lumen positive for cleaved-caspase-3 staining/area in the whole tissue positive for cleaved-caspase-3 staining) × 100. (B) Error bars indicate SEMs. (C) Error bars indicate SDs. *, P < 0.05; ***, P < 0.001. P values were determined by one-way ANOVA and Tukey's multiple-comparison test (B) and Mann-Whitney test (C).