FIG 4.

Viral titers and apoptosis in L cells following reovirus T1L and T3D-RV infection. (A) L cells were adsorbed with T1L or T3D-RV at an MOI of 1 PFU/cell, and viral titers were determined at the intervals shown by plaque assay. Viral titers are expressed as PFU per milliliter of cell homogenate. (B and C) L cells were adsorbed with T1L or T3D-RV at an MOI of 100 PFU/cell. (B) Cells were evaluated by AO assay at 38 hpi. The results are expressed as percentage of apoptotic cells per field of view. (C) Cell lysates were subjected to a Caspase-Glo 3/7 assay at 24 hpi. Caspase-3 activity is expressed in relative luminescence units. Data represent results from three independent experiments performed in triplicate. Error bars indicate SEMs. ****, P < 0.0001; one-way ANOVA and Tukey's multiple-comparison test.