FIG 6.

Viral proteins of BoHV-1 in tunneling nanotubes. KOP cells (A to C) and bovine fibroblasts (D to F) were infected at an MOI of 1 with BoHV-1-VP26-GFP for detection of GFP-tagged capsid protein VP26 (A and D), BoHV-1-gE-GFP for detection of GFP-tagged glycoprotein E (B and E), and BoHV-1-WT for detection of tegument protein US3 (C and F). At 12 h.p.i., the cells were fixed and additionally stained for F-actin (red) and nuclei (blue). Zoomed images of nanotubes (boxed regions) are in the right column. Yellow arrowheads indicate positions of viral proteins in nanotubes. Images were obtained using a Leica TCS Sp8 X confocal microscope.