FIG 6.

A PML-related function promotes ICP0-null virus replication and viral IE and E gene expression. HFF cells were treated with siRNAs specific for PML, a single siRNA from that pool (siRNA 06), or nontargeting siRNA and infected with 7134 virus at an MOI of 5. Samples were harvested at 4 to 10 hpi, and transcript levels of PML (A), ICP8 (B), and ICP27 (C) were analyzed by qRT-PCR and plotted relative to 18S rRNA levels. (D) Immunoblots of cell lysates obtained at 4 to 10 hpi, probed with antibodies specific for ICP8, ICP27, PML, and GAPDH. For statistical analyses for panels B and C, 2-way ANOVA including multiple comparisons was performed.